Activity 1: What is a zoonotic disease?

1. Define the word zoonotic with students. Write the word and its definition on the board.
2. Watch the video clip, How do viruses jump from animals to humans? Pause the video at 0:57 and emphasize the fact that most viruses are species-specific and don't move from species to species. However, when they do it can give way to deadly epidemics. Continue the video to learn more about the science of viral transmission from one species to another. 

Terms such as "Swine Flu" or "Bird Flu" can lead to the misconception that someone who consumes meat from a pig or eggs from a chicken (bird) could get the flu. Throughout the lesson, reinforce the science of virus transmission through live animal contact to correct these misconceptions.

Activity 2: Disease Transmission and Outbreak Simulation

Teacher Preparation: Half fill 10% of the cups with baking soda water. (For example, if there are 20 students, fill 2 cups with baking soda water). Half fill the remaining cups with distilled water. Place numbered nametags with the corresponding cup. Make note of which cups contain baking soda water. Set a Behavior Card under each cup. Pair the baking soda cups with a "Participants in multiple open, county, and state shows" card. Watch the instruction video below for more illustration.

Alternative Materials: Yellow food coloring (uninfected) and blue food coloring (infected) may be used instead of baking soda and phenol red. Any shade of green will represent infection at the end of the swapping activity, and may better demonstrate varying severity of illness. These materials may be easier to obtain.

1. Ask students if they remember where the swine flu was spread in the example used at the beginning of the video clip from Activity 1. (A county fair)
2. Provide the following explanation to students before beginning the simulation:
   • Some people and animals carry disease-causing pathogens (viruses or bacteria). Initially, these carriers appear healthy or show only mild signs of sickness. At some point they may get sick, but oftentimes they have already exposed and infected others. This activity is designed to simulate the uncontrolled spread of a disease through a population. Each of you will have a cup that is half-filled with water. One or two of you will have a cup that contains baking soda water. The students with the baking soda water will represent the original carrier(s) of the simulated disease. The original carrier(s) will make contact with other participants who will make contact with others to represent normal activities at a county fair. At the end of the simulation, everyone will be tested to see who has become infected and we'll discuss how to trace the infection back to its source.
3. Provide each student with one cup, the associated Behavior Card, the corresponding numbered nametag, and an Outbreak Investigation activity packet. 
4. Instruct students to put on their numbered nametag and review their behavior card. Tell them that they will be swapping fluids with other participants and each fluid swap should occur with a different person.
5. Ask students to move about the room and exchange fluids according to the instructions on their behavior card.
   • To exchange fluids, one person will dump all of the contents of their cup into the other person's cup.
   • Return half of the solution back to the empty cup. (Demonstrate with two water cups.)
   • Record the number of the person they exchanged with on their "Fluid Swap Record" in their packet.
6. Students should repeat step 4 as many times as specified on their behavior card. Remind students that each swap should be with someone they haven't already swapped with, and that they should only swap as many times as their behavior card indicates.
7. When swapping has finished, instruct students to return to their desks with their cups.
8. Add 2-3 drops of phenol red to each cup. A pink color change (bright or faint) indicates a positive result—this person is now considered "infected." No color change is "uninfected."
9. Work with the class to fill out Table 1 in their packets. Ask all with one exposure to raise their hands or stand. Discuss mild to severe infection and individuals who are at risk of severe complications (pregnant, very young or old, immunocompromised). Have the students answer questions 2 and 3 individually before discussing as a class.
10. Ask the students, "How did the number of people infected increase with each round of interactions?" Assuming one person was initially infected, fill out Table 4 and Graph 5 in the student in the student activity packet.
11. Have the students answer questions 6 and 7 individually and then discuss:
    • What do you notice about the rate of increase in the number of infections? (Discuss the doubling of number of infections in each round.)
    • Does the graph show linear or exponential growth? (exponential growth)
    • If we did this activity long enough, would everyone become infected? Why or why not? (Yes, but the doubling of infection we see in each generation does not continue indefinitely. In the beginning the curve increases exponentially, but then levels out. This pattern is known as logistic growth curve (S-curve). As the number of infected participants increases, it becomes increasingly more likely that an infected participant interacts with another participant that has already been infected. As a result, the number of new infections slows down. This type of growth also assumes that no one has any protection from the infection, such as vaccination.)
12. Ask the class, "How can we determine who was the original source of infection?" Discuss the idea that it's important for participants to realize that if they are infected, but can identify people they swapped with that AREN'T infected, then they could not have been the original source. Working through this should lead back to the people that were originally infected and those that they infected after the first swap.
13. After the discussion, have all "infected" participants stand up and "non-infected" participants remain seated. "Infected" participants should look around at "non-infected" participants. If anyone they exchanged fluids with is sitting, they should sit down as well. Continue this until there are 7-10 participants still standing. Direct the remaining participants to bring their list of people they swapped with to the front of the room and complete the "infection tree" as a class. Students have an "infection tree" in their packet that they can fill out as the group discusses. Completing the tree will require input from participants that remain seated.
14. Once the tree is complete, shade in all bubbles representing people that were "infected" at the end of the activity. Use this diagram to discuss and identify the probable initial source of the infection. Teacher Note: You will only be able to narrow it down to twice as many "suspects" as there were initial infected cups (e.g., starting with two cups means you'll be left with four suspects).
15. Activity Adaptation: Rather than using the "infection tree" to identify the initial source of infection, simply use the "stand up/sit down" method:
    • Have everyone stand up that is infected at the end of the activity.
    • If anyone standing up swapped fluids with anyone that is still sitting (uninfected), they should sit down.
    • Continue this until you're left with twice the number of people standing as you had initially infected cups.
    • One infected cup would result in two people left standing at the end. In this activity, there is no way to determine which person was the original source of infection, but you can share that information with the group.
16. Ask the class, "In a real investigation, how could we distinguish between who was the original source and who they infected? Why can't we do that here?" (In this activity, the potential first exposure to disease for everyone was the same when you were randomly handed a cup that either had water or a baking soda solution. You can't use earlier exposure or timeline to further narrow down who was originally infected and spread the "disease" to the rest of the group.)
17. Ask the students, "How would this investigation be different if you hadn't kept notes about whom you swapped fluids with and in what order?" Have them answer question 9 individually before discussing that one of the difficulties of a true outbreak investigation is that people's memories aren't always that great. It typically takes time for a disease to appear after initial exposure, and it takes even more time for an epidemiological investigation.
18. Ask, "What preventative measures could have been taken to avoid exposure to the disease?" (Lots of possible answers, including vaccination, attending fewer shows, etc.)
19. Ask the class, "How would an airborne disease spread differently? Why?" (In this activity, disease spread was dependent on a very direct, one-on-one interaction. Airborne diseases are able to spread more easily and indirectly from person to person, animal to animal, or animal to person and it often isn't clear when you've been exposed to someone with the disease. In this activity, it was very clear whom you swapped liquids with. It's much more difficult to track who you simply walk by, were in the barn with at the same time, etc.) 
20. Use a bubble blower and see who gets hit with a bubble to model airborne disease spread. Explain that airborne disease spread requires less movement. Some diseases can be transmitted in the air for long distances.

Activity 2: Animal Biosecurity Simulation

Teacher Preparation: Watch the instruction video to illustrate the activity. 

1. Explain to the class that the word biosecurity describes procedures intended to protect humans or animals against disease or harmful biological agents. They are going to simulate some common activities that might take place at a county fair livestock show.
   • If students are unfamiliar with livestock shows commonly held at county fairs, watch FFA & 4-H Students Show Skills at Junior National Livestock Show to gain familiarity.
2. Divide the class into small groups. Provide each group with a stuffed animal, feed samples, and a bucket. Place the show equipment on a table. Explain to the groups that they need to get their animal ready for a show by completing the following tasks:
   • "Inspect" the animal to make sure it is ready for the show.
   • Mix different feed samples in the bucket.
   • Collect the equipment to bring to the show.
3. Shine a black light on each student's hands to demonstrate how diseases can be spread by direct contact and from touching clothing, feed, buckets, equipment, fair food, etc. Demonstrate how pathogens can be spread by shoes by shining the black light throughout the room where students walked.
4. Discuss the importance of handwashing and how and when to wash hands to prevent the spread of disease.
   • Wet your hands with clean, running water (warm or cold), turn off the tap, and apply soap.
   • Lather your hands by rubbing them together with the soap. Lather the backs of your hands, between your fingers, and under your nails.
   • Scrub your hands for at least 20 seconds. Need a timer? Hum the "Happy Birthday" song from beginning to end twice.
   • Rinse your hands well under clean, running water.
   • Dry your hands using a clean towel or air dry them.
5. Have the students wash their hands and then use the black light again.
6. Invite the students to put on personal protective equipment (PPE) and repeat steps 4 and 5.
7. Discuss how wearing PPE helps to prevent disease transmission.
8. Use the information in the Background Agricultural Connections section to discuss ways to prevent direct contact, indirect contact, aerosol, oral, and vector-borne (infection transmitted to humans and other animals by blood-feeding anthropods, such as mosquitoes, ticks, and fleas) transmission.

Activity 4: Bring Home the Blue and Not the Flu! Interactive Modules

1. Direct students to the Bring Home the Blue, Not the Flu! online modules. Instruct them to complete each module:
   • Module 1: Introduction to Influenza, Zoonoses, and Disease Risks
   • Module 2: Zoonotic Disease Transmission
   • Module 3: Zoonotic Disease Prevention and Biosecurity
   • Module 4: Highly Pathogenic Avian Influenza (HPAI) Outbreak Case Study
   • Module 5: Influenza A Virus of Swine Origin (H3N2v) Outbreak Case Study
   • Module 6: One Health Agencies and Careers